为提升临夏地区冬季绵羊舍饲养殖同期发情效果及受胎率，支撑当地绵羊规模化、标准化养殖，在当地绵羊繁育场应用同期发情技术开展生产试验，探讨了不同时间注射同期发情激素药物、人工授精环境温度、兽用维生素 ADE对同期发情及受胎效果的影响。结果表明，撤栓前 1d注射快易孕注射用血清促性腺激素(PMSG)和氯前列醇钠注射液( PG)处理的绵羊同期发情率较撤栓当天注射药物处理增加 5.82~11.52个百分点。肌肉注射兽用维生素 ADE处理绵羊同期发情率为 98.88%，较未注射兽用维生素 ADE处理增加了 4.44~11.52个百分点；受胎率为 84.09%，较未注射兽用维生素 ADE处理增加了 6.44~14.35个百分点。撤栓前 1d肌肉注射 PMSG、PG，输精时保持输精枪、精液及操作台附近环境温度在 25 ℃左右的处理绵羊受胎率较未进行保温处理提高 5.70个百分点；联合肌肉注射兽用维生素 ADE进一步提高了绵羊受胎率，降低了无效养殖投入。因此，建议在北方绵羊繁育场冬季开展同期发情时，第 0天埋栓时肌肉注射兽用维生素 ADE 3 mL/只，第 11天肌肉注射 PMSG 330 IU/只、 PG 0.1 mg/只，第 12天早晨撤栓，第 14 d通过取暖措施保障输精枪、精液及操作台附近环境温度在 25 ℃左右，在 10:00时第 1次输精时肌肉注射促黄体素释放激素 A3 25 μg/只、兽用维生素 ADE 3 mL/只，并在 20:00时第2次输精，绵羊受胎效果较好。

To improve the estrus synchronization effect and conception rate of pen-fed sheep in winter in the Linxia area andto support large-scale and standardized sheep breeding locally, the production experiment was conducted at local sheep breedingfarms by using the estrus synchronization technology. The effects of hormone injection timing, artificial insemination environmentaltemperature, and vitamin ADE on estrus synchronization and conception were compared and analyzed. Results indicated that the es.trus synchronization rates of pregnant mare serum gonadotropin (PMSG) and cloprostenol sodium (PG) injection one day prior to sup.pository withdrawal increased by 5.82 to 11.52 percentage points compared with that of the treatment involving simultaneous injectionwith bolus withdrawal. The estrus rate of sheep treated with intramuscular injection of vitamin ADE was 98.88%, which was 4.44 to 11.52 percentage points higher than that of sheep without vitamin ADE injection, whereas the conception rate was 84.09%, which was 6.44 to 14.35 percentage points higher than that of sheep without vitamin ADE injection. The conception rate of sheep treated with in.tramuscular injection of PMSG and PG 1 d before suppository withdrawal and with the ambient temperature around the inseminationgun, semen, and operating table maintained at about 25 ℃ during insemination was 5.70 percentage points higher than that of sheepwithout warming treatment. Combined intramuscular injection of veterinary vitamin ADE further improved the conception rate of sheepand reduced ineffective breeding input. Therefore, it is recommended that, when conducting estrus synchronization in winter at sheepbreeding farms in northern China, veterinary vitamin ADE should be injected intramuscularly at 3 mL per sheep on Day 0 at Siliconeplugs insertion. PMSG 330 IU and PG 0.1 mg should be injected intramuscularly on Day 11. The Silicone plugs should be removed onthe morning of Day 12. On Day 14, heating measures should be adopted to maintain the ambient temperature around the inseminationgun, semen, and operating tableat about 25 °C, the first insemination at 10:00, gonadotropin-releasing hormone A3 at 25 μg and veteri.nary vitamin ADE at 3 mL should be injected intramuscularly, followed by the second insemination at 20:00. This protocol yields opti.mal conception results in sheep.

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甘肃省技术创新引导计划－东西协作专项( 23CXNN0007)；甘肃省技术创新引导计划－乡村振兴专项(24CXNN001、25CXNN011)；临夏州科技计划项目( 2025BN4026)。