为了探究暗期中断对不同光周期蔬菜作物光合特性的影响及品质调控效应，通过调控暗期光照模式，研究其对光周期响应型蔬菜生长发育及品质形成的调控作用，为设施蔬菜光环境精准调控提供依据。试验选取典型短日照作物蕹菜(台湾竹叶空心菜)与长日照作物叶用莴苣(紫萌生菜)为供试材料，日间采用 LED红蓝复合光(红∶蓝为 4∶1)进行 12 h的人工光照管理，光照强度为 200 μmol/(m2 · s)。夜间设置 3种暗期处理，即无光照中断( CK)、每 1h中断光照 5 min(夜间共中断 11次)、每 2h中断光照 5 min(夜间共中断 5次)，系统探究暗期中断对 2种蔬菜生长形态、光合特性、氮代谢过程及品质指标的影响规律。结果表明，暗期中断可显著促进长日照作物叶用莴苣的生长进程并提升品质，反之，对短日照作物蕹菜进行暗期中断处理显著抑制其生长，降低其品质，抑制效应随中断次数的增加呈增强趋势。暗期中断处理能够有效提高长日照作物叶用莴苣的净光合速率和电子传递速率，促进植株生长发育，增加可溶性蛋白、游离氨基酸等含氮化合物的含量，从而提升其营养品质，以每 1h中断 5 min的处理效果最优；短日照作物蕹菜在暗期中断处理下表现出生长抑制效应，不利于品质的形成和提升。本研究结果可为设施条件下蕹菜和叶用莴苣的高产优质栽培提供光环境精准调控的理论依据与技术支撑。

To explore the effects of dark period interruption on photosynthetic characteristics and quality regulation in vegetablecropswithdifferentphotoperiodicresponses,thisstudyaimedtoclarifytheregulatorymechanismofnight-breakongrowth,development,and quality formation of photoperiod-responsive vegetables, thereby providing a basis for the precise regulation of light environments inprotected vegetable production.We selected water spinach (Taiwanese Bamboo Leaf Water Spinach) and leaf lettuce (Zimeng Lettuce) asresearchmaterials,respectivelyrepresentingshort-daycropandlong-daycrop.Duringthediurnalperiod,theplantswereirradiatedwithLED Red-blue light (R:B=4:1) with intensity of 200 μmol/(m2·s) for 12 hours. 3 night-break treatments were set during night period: nolight interruption(CK), 5minutes of light interruption every 1hour(11times per night),and 5 minutes of light interruption every 2hours(5times pernight).The effectsof night-breakongrowth morphology, photosyntheticcharacteristics,nitrogenmetabolismprocess andquali.ty indexes were investigated with those two vegetables. Results showed that night interruption significantly promoted the growth processandimprovedthequalityoflong-day crop lettuce,where asnightinterruptiontreatmentsignificantlyinhibitedthegro wth andred uced the quality of short-day crop water spinach, with the inhibitory effect showing an increasing trend as the number of interruptions increased.Night interruption treatmenteffectively enhanced the net photosynthetic rate and electron transport rateof long-day crop lettuce, promot.ed plant growth and development, and increased the contents of nitrogen-containing compounds such as soluble protein and free aminoacids, thereby improving its nutritional quality, with the treatment of 5 min interruption per hour showing the optimal effect. Short-daycrop water spinach exhibited growth inhibition under night interruption treatment, which was unfavorable for quality formation and im.provement. These findings provide theoretical basis and technical support for precise regulation of light environment in high-yield andhigh-quality cultivation of wate rspinach and lettuce under protected cultivation conditions.

S636.2；S636.9

中国农业科学院科技创新工程( 34-IUA-03)；四川省自然科学基金( 2024NSFSC0397)；成都农业科技中心地方财政专项资金项目( NASC2022KR01、NASC2023ST06、NASC2024KR03)。